山西云顶山亚高山草甸优势种种间关系分析

为揭示山西云顶山亚高山草甸植物群落优势种的种间关系,本研究通过种间联结性和相关性测定,对其植物群落13个优势种78个种对的种间关系进行研究。结果表明:13个优势种间大部分种对的种间关联不显著,种间联结不紧密,说明山西云顶山亚高山草甸植物群落结构不很稳定,存在一定程度的生态退化风险;多数原生优势种在群落中占据主要位置,同时也出现少数非原生优势种优势明显,有逐渐取代典型原生优势种的逆行演替趋势,这是由于人类频繁的旅游扰动所致。因此,应当合理的控制旅游开发强度与游客数量,保持亚高山草甸生态系统的稳定与自我维持,以持续高效的维持云顶山亚高山草甸的经济及生态价值。     

Two Complete Mitochondrial Genomes From Leuctridae (Plecoptera: Nemouroidea): Implications for the Phylogenetic Relationships Among Stoneflies

The family-level relationships within Plecoptera have been a focused area of research for a long time. Its higher classification remains unstable, and the phylogenetic relationships within Plecoptera should be re-examined. Here, we sequenced and analyzed two complete mitochondrial genomes (mitogenomes) of Paraleuctra cercia and Perlomyia isobeae of the family Leuctridae. We reconstructed the phylogeny of Plecoptera based on 13 protein-coding genes (PCGs) from published stoneflies. Our results showed that the Bayesian inference and maximum-likelihood tree had similar topological structures except for the positions of two families, Peltoperlidae and Scopuridae. The Plecoptera is divided into two clades, the suborder Antarctoperlaria and the suborder Arctoperlaria. The two suborders subsequently formed two groups, Eusthenioidea and Gripopterygoidea, and Euholognatha and Systellognatha, which is consistent with the results of morphological studies. In addition, the Leuctridae is the earliest branch within the superfamily Nemouroidea. But the monophyly of Perloidea and Pteronarcyoidea are still not well supported.     

基于CNKI数据的农业物联网热点研究与分析

农业物联网技术在现代农业的发展历程中占有重要地位.通过关键词"农业物联网"在中国知网(CNKI)进行相关文献的采集和筛选,利用CiteSpace软件对所采集数据进行可视化分析,对国内农业物联网的研究热点进行分析与总结.结果表明:目前农业物联网的研究已进入快速发展阶段,但仍然有较大的研究空间可探索,需要诸多专家学者与科研单位进行合作和探究.随着国家对农业的重视以及乡村振兴战略的实施,农业物联网技术将会在农业生产中发挥巨大的作用.     

基于DNA条形码的红翅大小蠹和黄杉大小蠹分子鉴定

外来有害生物的快速准确鉴定有助于提高口岸检疫效率。红翅大小蠹(Dendroctonus rufipennis)和黄杉大小蠹(D. pseudotsugae)作为大小蠹属的非中国种是我国口岸原木检疫中频繁截获的检疫性有害昆虫。本研究以mtDNA COI基因5′端和3′端序列为标记对红翅大小蠹与黄杉大小蠹的11个样本开展分子鉴定有效性评价。结果显示,当以COI基因5′端和3′端为靶标时,两种大小蠹间的平均遗传距离分别为0.181和0.144,是种内遗传距离的60.3和48倍,且种内、种间遗传距离间无重叠现象,在系统发育树上都能聚为独立的分支,表明mtDNA COI基因5′端和3′端序列均可有效区分红翅大小蠹与黄杉大小蠹。     

燃煤电厂脱硫塔浆液起泡微观表征分析研究

利用彬长电厂和当涂电厂脱硫塔浆液起泡泡沫,通过X射线衍射法、傅里叶扫描电子显微镜法以及变换红外光谱法分析了吸收塔浆液泡沫样品的微观表征。结果表明:起泡和未起泡浆液在晶体形态、官能团上等具有相似的微观特征。但在形态结构上存在较大差异,起泡浆液的结构稳定性较差,存在使结构分裂的层间作用力。浆液结构变成杂乱无序的层状,其结晶水的含量也大幅度增加。     

Construction of high-density linkage map and identification of QTLs associated with resistance to black rot in radish (Raphanus sativus) by RAD sequencing

High-density marker-based QTL mapping can serve as an effective strategy to identify novel genomic information to facilitate crop improvement. In this study, we genotyped an F₂ population (KB12-1 × PP12-1) using a RAD-seq approach and constructed a high-density linkage map for radish. After a series of filtering procedures were performed, 17,124 SNPs and 3,336 indels with aa × bb genotyping were retained to obtain bin markers. Then, a linkage map comprising a total of 1,221 bin markers in nine linkage groups spanning 1,467.3 cM with an average marker interval of 1.2 cM was constructed. We evaluated the resistance of the F₂ mapping population to black rot using F₃ progeny, and two major QTLs related to black rot resistance were identified based on this map. Among these QTLs, qBRR2 on Chr.2 explained 26.97% of the phenotypic variation with a LOD score of 11.93, and qBRR7 on Chr.7 accounted for 27.06% of the phenotypic variation with a LOD score of 11.83. The additive effect of qBRR2 was positive (14.97); however, qBRR7 had the opposite effect (−11.99). The high-density linkage map and the major QTLs qBRR2 and qBRR7 provide new important information for disease resistance gene discovery and utilization in genetic improvement.     

不同施磷方式对海岛棉农艺性状及产量的影响

在新疆生态条件下,采用海岛棉新海35号研究同等用量3种施磷方式(犁地前全部撒施、犁地前撒施50%与作种肥施50%和全部作种肥施)对棉花农艺性状及产量的影响。结果表明:施磷方式对棉花生育进程的影响较小,各处理对新海35号棉花的农艺性状没有显著性的影响,P1处理可略为提高棉花花铃期叶面积指数,其中P1处理会略提高棉花后期的干物质积累量,以P1、P2处理的方式施磷肥,棉花的单株结铃数可提高4%~7%皮棉产量可提高12%~14.8%。     

传染性法氏囊病病毒主要结构蛋白VP2、VP1和VP2-VP1串联基因的原核表达及其初步应用

为获得传染性法氏囊病病毒(IBDV)特异性抗体检测用抗原VP2、VP1及VP2-VP1蛋白,分别设计引物扩增IBDV野毒株NN1172的VP2和VP1基因,并扩增VP2和VP1基因中抗原性和亲水性较好的重要区域,通过PCR扩增基因串联方法对截短的VP2和截短的VP1基因进行串联,首次获得VP2-VP1串联基因,并对VP2、VP1和VP2-VP1串联基因进行了原核表达和鉴定。结果成功构建了原核表达载体pET-VP2、pET-VP1和pET-VP2-VP1;诱导表达条件显示,3个重组质粒分别转入BL21菌株后经0.05 mmol/L IPTG诱导表达,分别得到分子量为69、114和63 kDa的VP2、VP1和VP2-VP1重组蛋白,且均以包涵体形式表达,3个重组蛋白分别于诱导后5、3和6 h时表达量最多。Western blot结果显示,表达的VP2、VP1和VP2-VP1蛋白与鸡抗IBDV阳性血清均具有良好的反应原性。以纯化的VP2、VP1和VP2-VP1蛋白作为包被抗原对传染性支气管炎病毒(IBV)、呼肠孤病毒(ReoV)、禽白血病病毒(ALV)和新城疫病毒(NDV)4种阳性血清检测均为阴性,表明所获得的纯化蛋白具有高度的特异性;对免疫了IBD灭活疫苗,IBD基因工程疫苗和IBD弱毒疫苗的商业鸡群进行抗体检测,结果均能显示疫苗免疫后机体抗体水平的变化趋势。本研究表明利用该原核表达系统所表达的3个蛋白均具有良好的免疫反应活性,为IBDV特异性抗体的检测和新型亚单位疫苗的研发奠定基础。     

添加剂NAM对盐碱地棉花产量和氮肥农学利用效率的影响

本研究旨在提高盐碱土壤中氮肥的利用率，降低硝化作用，减少氮素营养的流失。采用田间小区试验，设计处理：不施肥（CK）、常规施肥、常规施肥＋NAM（长效复合肥添加剂）、常规施肥量80%＋NAM，研究了不同处理盐渍土壤养分离子及棉花产量的变化。结果表明，肥料中加入NAM后，与常规施肥比较，可提高土壤有机质、碱解氮、速效钾和速效磷等养分含量，降低土壤碱化度，增加土壤阳离子交换量（Cation exchange capacity，CEC）及水溶性盐基离子K^＋、Ca^2＋与Mg^2＋含量。pH与电导率变化不明显。棉花产量提高了6.72%，氮肥农学利用效率提高了19.3%。常规施肥量80%＋NAM处理的棉花产量与常规施肥相当，但具有更高的肥料偏生产力和氮肥农学利用效率，可推荐在生产实践中应用。